The British Herpetological Society

The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.

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pdf 02. Immunocytochemical and quantitative study of interstitial cells in the High Mountain Toad Bufo bufo gredosicola during the spermatogenetic Cycle

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Open Access

pp.330-335

Authors: Benito Fraile, Ricardo Paniagua, Francisco J. Saez, & Marina C. Rodriguez And Miguel Lizana

Abstract: The interstitial cells of the toad Bufo Bufo gredosicola were studied throughout the seasonal period of spermatogenesis (from April to October) by means of immunocytochemical detection of testosterone and quantitative histological studies. The total number of interstitial cells per testis did not vary during the spermatogenetic period. However, in April, May and October, there were many interstitial cells showing an abundant testosterone content, whereas from June to September poorly-differentiated interstitial cells with a scanty testosterone content are the most abundant interstitial cell type. Since the interstitial cells with abundant testosterone content are larger than the interstitial cells with scanty testosterone content, the volume occupied by interstitial cells decreased in June-September. The development of thumb pads coincides with that of testosterone containing interstitial cells.

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