The Herpetological Journal
The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.
ISSN 0268-0130
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pdf 05. Enzyme (aldolase) activity in hyperosmotic media (NaCl and urea) in the terrestrial toad, Bufo viridis and frog Rana ridibunda
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pp.177-180
Authors: Gad Degani And Hani Hahamou
Abstract: ln this study we examined the adaptation of the enzyme Aldolase from frogs and toads to different temperatures and to hyperosmotic media of 400-1000mOsm/Kg of urea or NaCl . Maximum enzyme activity was found between 200-400m0sm/Kg NaCl, both in enzymes from green toads and from marsh frogs. However, a bove 500m0sm/Kg, the activity of enzymes from green toads was significantly higher than the activity of enzymes from marsh frogs. The activity of aldolase from green toads decreased very slowly as the media concentration of urea increased. However, the activity of aldolase from marsh frogs decreased rapidly under the same conditions. The maximum activity of aldolase from both frogs and toads was at 25°C. The activity of aldolase from green toads was significantly higher than the activity of aldolase from marsh frogs when measured only at high temperatures (35°C). Th e results of this study support the idea that the biochemical systems of terrestrial amphibia are tolerant to hyperosmotic media
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