The Herpetological Journal
The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.
ISSN 0268-0130
2021 Impact Factor from Clarivate for the Herpetological Journal is 1.194, an increase of 0.332 from 2020.
pdf 03. Buccal swabs as a non destructive tissue sampling method for DNA analysis in amphibians
1227 downloads
Open Access
pp.175-178
Authors: Nathalie Pidancier , Christian Miquel , Claude Miaud
Abstract: This study describes a non-destructive DNA sampling method for genetic studies on amphibians using buccal swabs. We assessed the quantity and quality of DNA collected in each species by amplifying a part of the cytochrome b gene (381-1060 bp) and microsatellite markers . . Buccal swab sampling is a useful alternative method for DNA sampling for both mtDNA and nDNA markers in amphibians. However, only frozen storage allowed microsatellite genotyping. We conclude that this method could greatly increase the accessibility of genetic studies in small vertebrates and could be preferred in the field of conservation genetics.
Keywords: sampling, mtDNA, nDNA, conservation genetics
Download Access:
The latest 8 issues can be downloaded when logged in with a Herpetological Journal subscription membership.
Individual articles can be purchased for download.
Older issues and occasional Open Access articles are available for public download
Submissions:
For further information and submission guidelines please see our Journal Instructions to Authors
Latest Issue
IMPORTANT NOTE - JUNE 2020
Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.
Aligning with this change, it is now no longer possible to purchase a subscription that includes a print copy of the HJ. All members who have existing HJ print subscriptions that remain active as at end June 2020 will receive the full four 2020 print editions. New subscribers or renewals after this time will only have option to subscribe to the online only subscription package. Subscription pricing has been amended to reflect the content changes.