The Herpetological Journal
The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.
ISSN 0268-0130
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pdf 03. Buccal swabs as a non destructive tissue sampling method for DNA analysis in amphibians
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Open Access
pp.175-178
Authors: Nathalie Pidancier , Christian Miquel , Claude Miaud
Abstract: This study describes a non-destructive DNA sampling method for genetic studies on amphibians using buccal swabs. We assessed the quantity and quality of DNA collected in each species by amplifying a part of the cytochrome b gene (381-1060 bp) and microsatellite markers . . Buccal swab sampling is a useful alternative method for DNA sampling for both mtDNA and nDNA markers in amphibians. However, only frozen storage allowed microsatellite genotyping. We conclude that this method could greatly increase the accessibility of genetic studies in small vertebrates and could be preferred in the field of conservation genetics.
Keywords: sampling, mtDNA, nDNA, conservation genetics
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