The Herpetological Journal
The Herpetological Journal is the Society's prestigious quarterly scientific journal. Articles are listed in Biological Abstracts, Current Awareness in Biological Sciences,Current Contents, Science Citation Index, and Zoological Record.
ISSN 0268-0130
2021 Impact Factor from Clarivate for the Herpetological Journal is 1.194, an increase of 0.332 from 2020.
pdf 03. Identification of Bufo larvae by molecular methods
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Open Access
pp.145-148
Authors: Louise Bardsley, Sean Smith And Trevor J . C. Beebee
Abstract: We have characterized two molecular techniques, protein typing and RAPD analysis, for the identification of two species of European Bufo larvae (Bufo bufo and B. calamita). These tadpoles are very difficult to distinguish on morphological grounds. Protein typing was improved by the use of a sensitive (silver-based) staining method, and replacement of low temperatures with ethanol submergence for tissue storage. Both techniques reliably resolved the two species and required very small amounts (<2 mg) of tissue, which could be obtained easily and without sacrificing the animals.
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Please note that as from Volume 31 Number 1 (January 2021) on, the Herpetological Journal will be available as an online publication only - the last print edition will be Volume 30 Number 4.
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